Product Informations

• Goods Code BD-PT5163
• AnotherName APLN; APEL; Apelin; APJ endogenous ligand
• Goods Name Apelin Rabbit Polyclonal Antibody
• Category 493
• Gene Name APLN
• Protein Name Apelin
• Recommended Applications WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• Reactivity Human,Mouse,Rat
• Concentration 1 mg/ml
• Storage Buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Immunogen The antiserum was produced against synthesized peptide derived from the C-terminal region of human APLN. AA range:28-77
• Specificity Apelin Polyclonal Antibody detects endogenous levels of Apelin protein.
• Dilution Ratio WB 1:500-1:2000, IHC-P 1:100-300, ELISA 1:20000, IF-P/IF-F/IF-ICC 1:100-300, Not yet tested in other applications.
• Predicted Molecular Weight 16kDa
• Shipping and Storage Conditions -20°C/1 year
• Host Rabbit
• Isotype Rabbit,IgG
• Background This gene encodes a peptide that functions as an endogenous ligand for the G-protein coupled apelin receptor. The encoded preproprotein is proteolytically processed into biologically active C-terminal peptide fragments. These peptide fragments activate different tissue specific signaling pathways that regulate diverse biological functions including fluid homeostasis, cardiovascular function and insulin secretion. This protein also functions as a coreceptor for the human immunodeficiency virus 1. [provided by RefSeq, Feb 2016],
• Cellular Localization Secreted . Secreted, extracellular space . Abundantly secreted in the colostrum. Lower level in milk. Decreases rapidly within several days after parturition in milk, but is still detectable even in commercial milk. .
• Function function:Endogenous ligand for APJ, an alternative coreceptor with CD4 for HIV-1 infection. Inhibits HIV-1 entry in cells coexpressing CD4 and APJ. Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives. The oral intake in the colostrum and the milk could have a role in the modulation of the immune responses in neonates. May also have a role in the central control of body fluid homeostasis by influencing AVP release and drinking behavior.,PTM:Several active peptides may be produced by proteolytic processing of the peptide precursor.,similarity:Belongs to the apelin family.,tissue specificity:Expressed in the brain with highest levels in the frontal cortex, thalamus, hypothalamus and midbrain. Secreted by the mammary gland into the colostrum and the milk.,
• Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Lead Time In stock
• Clonality Polyclonal
• Human Gene ID 8862
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=8862
• Human Swissprot No. Q9ULZ1
• Human Swissprot Link http://www.uniprot.org/uniprotkb/Q9ULZ1/entry
• Mouse Gene ID 30878
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=30878
• Mouse Swissprot No. Q9R0R4
• Mouse Swissprot Link http://www.uniprot.org/uniprot/Q9R0R4
• Rat Gene ID 58812
• Rat Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=58812
• Rat Swissprot No. Q9R0R3
• Rat Swissprot Link http://www.uniprot.org/uniprot/Q9R0R3

Picture

	Western blot analysis of lysate from 22RV-1 cells, using APLN Antibody.
	Western Blot analysis of 22RV-1, H460, U87, mouse brain cells using Apelin Polyclonal Antibody.. Secondary antibody was diluted at 1:20000
	Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100
	Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100
	Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.

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