Product Informations

• Product No. BD-PD400145
• Product Name SARS-CoV-2 (2019-nCoV) Spike Neutralizing Antibody, Rabbit Mab
• Category 621
• Recommended Applications ELISA,FCM,Neutralization
• Concentration ELISA 0.5-1 μg/mL
• Storage Buffer 0.2 μm filtered solution in Histidine and Arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH6.0
• Immunogen Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Protein
• Appearance Liquid
• Endotoxin < 3 EU/mg
• Host Monoclonal Rabbit IgG Clone #001
• Conjugate Unconjugated
• Purification Protein A

Picture

	Serial dilutions of Anti-SARS-CoV-2 Neutralizing Antibody was detected by SARS-CoV-2 (2019-nCoV) Inhibitor Screening ELISA Kit. The IC50> (WT) is typically 0.5-5 nM.
	The neutralization activity is measured by microneutralization assay in vitro. The virus microneutralizaiton (MN) test was performed on 293T-ACE2 cells infected with SARS-CoV-2 Spike Pseudovirus, or SARS-CoV-2 Spike Omicron (B.1.1.529) Pseudovirus, or SARS-CoV-2 Delta (B.1.617.2) variant Spike Pseudovirus, under treatment of serial dilutions of neutralizing antibody. The infection was neutralized by increasing concentrations of Anti-SARS-CoV-2 Neutralizing Antibody. Rate of inhibition was determined by comparing the Relative Light Unit (RLU) of Luciferase reporter in different antibody concentrations. IC50(WT)is 0.29 µg/mL. IC50(Delta) is 0.15 µg/mL. No neutralization activity to SARS-CoV-2 Spike Omicron (B.1.1.529) Pseudovirus
	Flow cytometric analysis of SARS-COV-2 Spike overexpressed HEK293 Cells were stained with purified anti-SARS-COV-2 Spike Neutralizing Rabbit MAb, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.\ (Validation Experiment)

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