Plant Direct Expansion TaqMan qPCR Kit

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GoodsCode: BDAG0051

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Specs	Price	Futures	Quantity

1ml

50.00

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5ml

210.00

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100ml

3070.00

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Product Informations

Goods Code BDAG0051
Goods Name Plant Direct Expansion TaqMan qPCR Kit
Category 601
Instruction Manual /files/uploads/instructions/2024/202403/BDAG0051_植物直扩TaqMan qPCR试剂盒_说明书.pdf

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Product Details

Reagent components

Component name	Item number
Component name	BDAG0051-1ml	BDAG0051-5ml	BDAG0051-10ml
2×Plant Direct TaqMan qPCR Master Mix	1 mL	5 mL	10 mL
Buffer RLB18 ROX ReferenceⅠ (50×) * ROX Reference Ⅱ (50×) *	25 mL 40 µL 40 µL	125 mL 200 µL 200 µL	2,500 mL 400 µL 400 µL

*Note: ROX Reference is an optional reagent and is not included in this product. The usage methods for different models of Real-Time PCR instruments are as follows:
1. ROX Reference Ⅰ(50×) is a high-concentration ROX and is applicable to the following models of Real-Time PCR instruments:
- Applied Biosystems 7300 Real-Time PCR System(Thermo Fisher Scientific);
- Applied Biosystems 7900 Real-Time PCR System(Thermo Fisher Scientific);
-Step One Plus Real-Time PCR System (Thermo Fisher Scientific).
2. ROX Reference II (50×) is a low-concentration ROX and is applicable to the following models of Real-Time PCR instruments:
- AppliedBiosystems 7500 Real-Time PCR System (Thermo Fisher Scientific);
- AppliedBiosystems 7500 FAST Real-Time PCR System (Thermo Fisher Scientific)
3. When used with the following models of Real Time PCR amplifiers, ROX Reference is not required:
Roche, Bio-Rad, TaKaRa, Hongshi, Yarui, Borui, Tianlong and other Real-Time PCR instruments that do not require the addition of ROX calibration dyes.

Product Description
This product can be used for various types of plant samples without the need for nucleic acid extraction. It can directly conduct TaqMan Probe qPCR reactions for the detection of gene content and transgenic organisms, etc. The plant samples were crushed using the specially developed Buffer RLB18 component in the kit, and then the qPCR amplification reaction was directly carried out.
The product employs a directionally modified and antibody-blocked direct-expansion Hot-Start DNA Polymerase (HOT-Start DNA Polymerase), combined with an optimized hot-start qPCR dedicated buffer system, which has extremely strong tolerance to PCR inhibitors in various samples. It can effectively avoid non-specific amplification reactions similar to those caused by non-heat-activated polymerases, thereby enhancing the efficiency of PCR amplification. It can also achieve ideal amplification for polysaccharide and polyphenol samples and obtain highly sensitive qPCR detection results.

Product features
1. There is no need for nucleic acid extraction or purification. qPCR reactions can be directly conducted on various plant samples, saving time and effort.
2. In combination with the specially developed high-performance sample pretreatment component Buffer RLB18, the direct qPCR reaction effect can be comparable to that of the product extracted from the same sample volume as the template.
3. The use of antibody-blocked mutant polymerase enables Hot Start PCR reactions. When combined with a specially developed Buffer system, it features high amplification efficiency and high amplification sensitivity.
4. It is capable of amplifying samples of polysaccharides and polyphenols.

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