Mouse IgG ELISA Kit

GoodsCode: BDEL-0311
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  • 48T
    210.00
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    96T
    360.00
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Product Informations

  • Product No. BDEL-0311
  • Synonyms Mouse IgG ELISA Kit
  • Product Name Mouse IgG ELISA Kit
  • Category 595
  • Instruction Manual /files/uploads/instructions/2024/202407/BDEL-0311_Mouse IgG ELISA Kit_说明书.pdf

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Product Details

本试剂盒采用双抗体夹心ELISA法。抗小鼠IgG total单抗包被于酶标板上,标本和标准品中的IgG会与单抗结合,游离的成分被洗去。加入生物素化的抗小鼠IgG total抗体和辣根过氧化物酶标记的亲合素。生物素与亲合素特异性结合;抗小鼠IgG total抗体与结合在单抗上的小鼠IgG结合而形成免疫复合物,游离的成分被洗去。加入显色剂,若反应孔中有小鼠IgG total,辣根过氧化物酶会使无色的显色剂现蓝色,加终止液变黄。在450nm处测OD值,小鼠IgG total浓度与OD450值之间呈正比,可通过绘制标准曲线求出标本中小鼠IgG total浓度。
 

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