Protein G Gravity Preloaded Column Kit AbPur rProtein G Kit

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GoodsCode: BDTL0003-K

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Product No. BDTL0003-K
Product Name Protein G Gravity Preloaded Column Kit AbPur rProtein G Kit
Category 530
Instruction Manual /files/uploads/instructions/2023/BDTL0003-K_蛋白G重力预装柱套装_说明书.pdf

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组分名称	规格	数量
蛋白G重力预装柱	3ml	1
结合/洗杂Buffer	100ml	2
洗脱Buffer	100ml	1

本试剂盒提供1根填装3ml蛋白G层析介质的重力预装柱，2瓶100ml的结合/洗杂Buffer和1瓶100ml的洗脱Buffer。不需要再进行填料填装和缓冲液配制。整个纯化流程大约需要30min（主要取决于样品体积和溶液的粘稠性），操作快捷。蛋白G层析介质是用于分离和纯化lgG的亲和层析介质，具体性能见下表。Protein G是一种分离自G Streptococci的细胞壁蛋白，它可通过其Fc片段结合哺乳动物IgG。重组protein G含有高亲和结合位点，减少了非特异性吸附。Protein G和Protein A有不同的lgG结合特性，相比Protein A， Protein G对牛、羊、马等多克隆抗体有更强的结合力，它还可以结合不能与Protein A很好结合的大鼠lgG、人lgG3和小鼠lgG1。

蛋白G层析介质性能表

指标	性能
基质	4%琼脂糖微球
配体	重组蛋白G
载量	>30mg 人lgG/ml 介质
粒径 (um)	45-165
最大流速	0.1 MPa, 1 bar
pH 稳定范围	3-12
储存缓冲液	20% 乙醇
储存温度	2℃ - 8℃

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Q1: What is the maximum sample volume that can be added to the 3ml gravity pre-packed column set at one time?

Q2: What is the centrifugal force for the resin?

Q3: I didn't enrich antibodies in my purification. Can the flow-through, wash, and eluate be passed through the column again?

Q4: For G-column purification, is there a pH requirement for the 6M guanidine hydrochloride solution used to wash the column?

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