Product Introduction
The ChIP (Chromatin Immunoprecipitation) detection kit, also known as the chromatin immunoprecipitation detection kit, is the most crucial step in the CHIP-related experimental process. ChIP is a process in which proteins and DNA form complexes through formaldehyde fixation in living cells. Then, the complexes are randomly cut into small chromatin fragments of a certain length range by ultrasound. Subsequently, the DNA fragments bound to the target protein are enriched through antigen-antibody specific binding reactions. After cross-linking, the DNA is separated and purified. The obtained DNA is detected and analyzed to obtain information on the interaction between proteins and DNA.
This kit includes Box A, Box B and Box C, allowing for 24 independent chromatin immunoprecipitation (ChIP) reactions. The provided buffer is sufficient for preparing chromatin from six 15 cm plates of cultured cells, with each plate capable of providing up to 10 chromatin preparations (varying depending on the type of cell and assay). The testing period is approximately 2 to 3 days. It has been verified that the rProtein A/G MagPoly Beads provided in the kit can better immunoprecipitate chromatin. After protein-DNA cross-linking, DNA is purified using the centrifugal column method, which is convenient and fast, without the need for phenol/chloroform extraction and ethanol precipitation. Objective: The enrichment of DNA sequences can be analyzed through various methods such as standard PCR, quantitative real-time PCR or sequencing.