Product Informations

• Product No. BDAA0260
• Product Name HRP-Nanoantibody anti Mouse for IP
• Category 502
• Recommended Applications WB,mIHC,IP
• Reactivity Mouse
• Specificity This antibody is immunoblotting reagents that enable the trouble-free detection of immunoblotted target protein bands, without interference from denatured IgG. This allows to detect the (co-)immunopre
• Dilution Ratio WB 1:2000-10000, IP 1:5000-20000, mIHC 1:200-500
• Shipping and Storage Conditions 2°C to 8°C/1 year
• Host Camel,VHH
• Isotype Mouse IgG1/2a/2b/3 Kappa

Picture

Immunoprecipitating GAPDH in Hela whole cell lysate with mouse anti GAPDH antibody. Western blot was performed on the immunoprecipitate using anti-GAPDH antibody at 1:5000 dilution, and followed by the AbFlour 800 labeled anti-Mouse IgG antibody at 1:5000 dilution. Lane 1 (Input): 20ug Hela whole cell lysate. Lane 2 (-):Mouse monoclonal IgG1 Isotype Control instead of GAPDH antibody in Hela whole cell lysate. Lane 3 (+): 6ug GAPDH antibody IP in 1000ug Hela whole cell lysate

Mouse IgG for IP was diluted at 1:3000 to detect non-reduced or reduced different forms of mouse IgG, 1ug/lane

Product Details