Product Informations
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Product No.
BD-PA0005
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Synonyms
ARNIP; CHIMP; RNF199; RCHY1
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Product Name
Pirh2 Mouse Monoclonal Antibody
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Category
493
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Gene Name
Pirh2
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Recommended Applications
WB,IHC-P,ICC,FCM,Elisa
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Reactivity
Human,Rat
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Storage Buffer
Ascitic fluid containing 0.03% New type preservative N.
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Immunogen
Purified recombinant fragment of human Pirh2 expressed in E. Coli.
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Dilution Ratio
WB 1:500-1:2000, IHC-P 1:200-1:1000, ICC 1:200-1:1000, FCM 1:200-1:400, ELISA 1:10000
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Reference Molecular Weight
30kDa; 60kDa (homodimer)
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Shipping and Storage Conditions
4℃; -20℃ for long term storage. Avoid freeze/thaw cycles.
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Host
Mouse
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Isotype
IgG1
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Clone No.
1H10
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Lead Time
Three working days
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Clonality
Monoclonal
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Human Gene ID
25898
Picture
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Western blot analysis using Pirh2 mouse mAb against Hela (1), A549 (2), MCF-7 (3) and PC-12 (4) cell lysate. |
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Immunofluorescence analysis of Hela cells using Pirh2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. |
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Flow cytometric analysis of PC-12 cells using anti-Pirh2 mAb (blue) and negative control (red). |
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Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using anti-Pirh2 mouse mAb |
Product Details
Pirh 2 (P53 induced RING-H2 protein), also known as RCHY1, it forms dimers through its N- and C-terminus in cells. The Pirh2 has ubiquitin-protein ligase activity and it binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. The Pirh2 is oncogenic because loss of p53 function contributes directly to malignant tumor development. Pirh2 expression decreases the level of p53, and a decrease of endogenous Pirh2 expression increases p53 levels. Pirh2 is therefore considered, together with MDM2, to act as a negative regulator of p53 function.
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