Product Informations

• Product No. B1032
• Product Name PCNA (12D10) Mouse Monoclonal Antibody
• Category 488
• Recommended Applications WB,IF-P,IF-F,IF-ICC,IHC-P,Elisa
• Reactivity Human,Rat,Mouse
• Concentration 1 mg/ml
• Storage Buffer Liquid in PBS,pH 7.4, containing 50% glycerol，2% BSA and New type preservative N.
• Immunogen Synthetic Peptide of PCNA
• Specificity The antibody detects endogenous PCNA protein.
• Dilution Ratio WB 1:5000, IHC-P 1:200, IF-P/IF-F/IF-ICC 1:200
• Reference Molecular Weight 29KD
• Host Mouse
• Background PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is acofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesisduring DNA rep
• Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
• Lead Time In stock

Picture

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,PCNA Monoclonal Antibody(12D10) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Product Details