Product Informations

• Product No. BD-PD246748
• Synonyms IgG2 Protein, Human
• Product Name Human IgG2-Fc Protein (257 Ser/Ala)
• Category 466
• Shipping Method In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Reactivity Human
• Storage Buffer Lyophilized from sterile PBS, pH 7.4 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information
• Reference Molecular Weight The recombinant human IgG2-Fc consists of 228 amino acids and has a predicted molecular mass of 25 kDa. As a result of glycosylation, the apparent molecular mass of IgG2-Fc is approximately 34 kDa in SDS-PAGE under reducing conditions.
• Predicted N-terminus Glu 99
• Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
• Biological Activity Measured by its ability to bind human CD32a-His (Cat:10374-H08H1) in a functional ELISA.
• Host HEK293 Cells
• Precautions A DNA sequence encoding the human IgG2 Fc region (P01859) (Glu 99-Lys 327, 257Ser/Ala) was expressed and purified.
• Background Immunoglobulin heavy constant gamma 2
• Purification > 90 % as determined by SDS-PAGE
• Human Swissprot No. P01859

Picture

Product Details