Q1: Why do different batches of Biodragon Hybridoma Feeder Additive have different colors?
The color difference is due to the fact that both our cryopreservation solution and the addition factor contain phenol red. During the packaging process, the liquid reacts with co2 in the atmosphere to become acidic, which will cause a color change. However, it has no impact on the product quality. Phenol red is an acid-base indicator
Q2: How many hybridoma addition factors are needed for a 96-well plate
How many hybridoma addition factors are needed for a 96-well plate
Q3: For the Hybridoma Feeder Additive, is it needed during the fusion stage and at half-medium change?
It needs to be added continuously.
Q4: How much Hybridoma Feeder Additive is needed for one 96-well plate?
Each well is approximately 200 μ l, and for 96 Wells, it's about 20 mL. At a ratio of 1:10, approximately 2ml of hybridoma additive factor is used. Considering that the usage varies among different customers, approximately 1-2ml of hybridoma additive factor is needed for one 96-well plate
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