Q1: The homology between human and mouse EGF is very low, only 55%. But you have verification data in mouse, so can it be used in mouse?
Yes, it's okay. This method is the one used in the pharmacopoeia and is fine. At present, this method is basically adopted in the industry.
Homology is not the only criterion for judgment. This is the case for all human cytokines with mouse data. Even if the homology is low, they can still be used.