The manual provides the steps for in vitro bioluminescence experiments and does not require lysis. If you need to conduct in vitro reporter gene testing, you will need to lyse cells. For specific steps, please refer to the website and make modifications according to your specific needs.

The manual provides the steps for in vitro bioluminescence experiments and does not require lysis. If you need to conduct in vitro reporter gene testing, you will need to lyse cells. For specific steps, please refer to the website and make modifications according to your specific needs.

What is the difference between sodium and potassium D-luciferin salts?

There is basically no difference between the two, but there may be some minor differences in physical properties. For example, the sodium salt of D-luciferin is slightly more granular and has better water solubility than the potassium salt. In addition, according to the reported literature, about three times more of the literature uses potassium salts. Many researchers have a preference for potassium salts in their bodies, but the effect of any one is consistent.