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1. Similarities: All are medium-pressure pre-assembled columns. Medium-pressure pre-assembled columns cannot be disassembled and are not suitable for gravity pre-assembled columns. If the customer wants to use it for gravity prefabricated columns, they can choose the corresponding product for non-medium-pressure prefabricated columns 2. Differences The fillers of the two products are different. The pre-packed column of Ni NTA high-speed chromatography medium (6FF) is filled with Ni NTA Beads 6FF. Ni NTA is resistant to low concentrations of reducing agents and chelating agents, but not resistant to the simultaneous addition of multiple reagents. It is suitable for conventional his tag affinity purification. The Ni chromatography medium (6FF) pre-packed column is filled with Ni Smart Beads 6FF. Ni Smart has a relatively high tolerance to reagent concentration and is suitable for purification under special conditions or for the purification of eukaryotic secreted expression proteins.

First, use the original eluent concentration. You can check the result after the first test. If the effect is not good, you can change the imidazole gradient elution. If the effect is not good, it means that the purity of the protein after elution does not meet expectations. Imidazole gradient elution involves first eluting with diluted low-concentration imidazole to remove impurity proteins, and then eluting with high-concentration imidazole to obtain the target protein.