1xTAE buffer, with a voltage drop of 5V/cm (the voltage across the terminals divided by the distance cm between the two platinum wires). The electrophoresis time depends on the length of the gel. It is best if all the bands are completely separated. Generally, the yellow dye stops when it moves to the positive terminal of the glue. Set the constant pressure mode for electrophoresis. After setting a constant voltage, current will automatically come out.

There is no 1% of the experimental graph. The electrophoresis effect of marker I with 1%Agarose gel is not good because the resolution of 1%Agarose gel is too low. The other three can be made with 1% glue.

5ul