FAQ

可感染细胞的假病毒目前主要由HIV骨架和VSV骨架两种，两种病毒都可以用于细胞实验和动物实验，VSV骨架假病毒做动物实验效率会略好一些。我们的假病毒是HIV骨架的。
  HIV骨架假病毒三个质粒=慢病毒(HIV)的包装质粒+新冠S蛋白包膜外壳+载体质粒（含有GFp和荧光素酶）
  VSV骨架假病毒三个质粒=水泡性口炎病毒载体（VSV）的包装质粒+新冠S蛋白包膜外壳+载体质粒（含有GFp或荧光素酶）
  假病毒的动物实验：先用腺病毒让动物的部分组织或器官有ace2细胞的过表达，在用可感染细胞的假病毒进行侵染实验

类别	产品情况	货号	名称
原始株	有	BDAA0026	FNV-SARS-CoV-2-S（可感染细胞）
Alpha	无
Beta	只有三个点突变株（E484K、K417N、N501）	BDAA0084	FNV-SARS-CoV-2-S(可感染细胞)(E484K、K417N、N501Y)
Gamma	只有三个点突变株（E484K、K417N、N501）	BDAA0084	FNV-SARS-CoV-2-S(可感染细胞)(E484K、K417N、N501Y)
Delta	有	BDAA0115	FNV-SARS-CoV-2-S(可感染细胞)(B.1.617.2)(delta)
Lambda	无
Omicron	有	BDAA0117	FNV-SARS-CoV-2-S(可感染细胞)(B.1.1.529)(Omicron)

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mock是空细胞

The pseudovirus contains no COVID-19 nucleic acid at all. It only has S protein on its surface and is not replicable. It can be used in laboratory cell rooms and is not pathogenic. What is written in our instruction manual is relatively conservative.

Our pseudovirus of the novel coronavirus has been tested on animals, but the results were not good and it could not be detected. It is not recommended for infecting mice.

The current situation of pseudoviruses that can infect cells

PCR quantitative primers

Is the fluorescence single fluorescence or double fluorescence

Product color

How are pseudoviruses that can infect cells constructed

What kit is used to activate the luciferase in pseudoviruses?

What is "mock" in the instruction manual

Is the pseudovirus the skeleton of HIV

Can our pseudovirus that can infect cells infect Vero cells?

The dosage per well of the cells to be infected

Dosage for infectable cells

The pseudovirus that can infect cells can be detected by using the S protein reagent for detecting COVID-19, right?

In neutralization assays, how much reduction is considered effective?

The value of luciferase

Gradient dilution method, greater than 1 × 10 to the power of 7. We use the gradient dilution method. We use the same number of cells. For example, if the number of cells is all 1E+5, if 10ul of the virus is added and all the cells are fully infected, it is overinfection. If 1ul is added and 10% of the cells are infected, then 1ul of the virus has infected 1E+4 cells. Therefore, 1ml can infect 1E+7 cells. It's roughly calculated like this.

FAQ

Q1: The current situation of pseudoviruses that can infect cells

Q2: What is the 3.8k gene sequence or primer sequence of the S gene in a pseudovirus with infectious activity

Q3: What is "mock" in the instruction manual

Q4: Can it be used in a laboratory cell culture room? Any harm to the laboratory and personnel?

Q5: Can the cell-infecting COVID-19 pseudovirus infect mice?

Q6: The current situation of pseudoviruses that can infect cells

Q7: PCR quantitative primers

Q8: Is the fluorescence single fluorescence or double fluorescence

Q9: Product color

Q10: How are pseudoviruses that can infect cells constructed

Q11: What kit is used to activate the luciferase in pseudoviruses?

Q12: What is "mock" in the instruction manual

Q13: Is the pseudovirus the skeleton of HIV

Q14: Can our pseudovirus that can infect cells infect Vero cells?

Q15: The dosage per well of the cells to be infected

Q16: Dosage for infectable cells

Q17: The pseudovirus that can infect cells can be detected by using the S protein reagent for detecting COVID-19, right?

Q18: In neutralization assays, how much reduction is considered effective?

Q19: The value of luciferase

Q20: What is the titer?